In this study, we used individual trip data to estimate a macroscopic fundamental diagram (MFD) that relates flow (or production) to density (or state) in Daegu metropolitan city. The individual trip data were generated by processing data that were collected from DSRC-based (dedicated short range communication) traffic data collection system. Using the processed individual trip data, we first examined whether the assumptions for MFD are valid, and then the relation between outflow and accumulation was estimated in our study site. As a result, we found that i) the assumptions are valid to construct MFD; and ii) the reproducible and well-defined MFDs exist in the network level.